 | The Lion King at the Hollywood Bowl (2025) as Self |
 | Spellbound (2024) as Luno - Oracle of the Moon (voice) |
 | Dicks: The Musical (2023) as Harris |
 | Once Upon a Studio (2023) as Timon (voice) |
 | Finally Home: Making Beau is Afraid (2023) as Self |
 | Beau Is Afraid (2023) as Roger |
 | Too Soon: Comedy After 9/11 (2021) as Self |
 | Elegies for Angels, Punks and Raging Queens (2020) as Giacomo |
 | Bully. Coward. Victim. The Story of Roy Cohn (2019) as Self |
 | Every Act of Life (2018) as Self |
 | The Vanishing of Sidney Hall (2018) as Harold |
 | National Theatre Live: Angels In America — Part Two: Perestroika (2017) as Roy M. Cohn/The Angel Antarctica |
 | National Theatre Live: Angels In America — Part One: Millennium Approaches (2017) as Roy M. Cohn/Prior 2 |
 | Carrie Pilby (2017) as Dr. Petrov |
 | No Pay, Nudity (2016) as Hershel Thalkin |
 | Mike Nichols: An American Master (2016) as Self |
 | The Nance (2014) as Chauncey Miles |
 | Showing Up (2014) as Self |
 | Mel Brooks: Make a Noise (2013) as Self |
 | The English Teacher (2013) as Carl Kapinas |
 | Elaine Stritch: Shoot Me (2013) as Himself |
 | Mirror Mirror (2012) as Brighton |
 | Pride of The Lion King (2011) as Self |
 | The Nutcracker (2010) as Uncle Albert |
 | I'm Still Here (2010) as Nathan Lane (uncredited) |
 | Astro Boy (2009) as Hamegg (voice) |
 | Waking Sleeping Beauty (2009) as Self (archive footage) |
 | A Muppets Christmas: Letters to Santa (2008) as Officer Meany |
 | Swing Vote (2008) as Art Crumb |
 | The Roots of 'Hairspray' (2007) as Self (voice) |
 | Trumbo (2007) as Self |
 | ShowBusiness: The Road to Broadway (2007) as Self |
 | The Dish on Dolls (2006) as Stagehand in Jacqueline Susann's Valley of the Dolls (archive footage) |
 | The Producers (2005) as Max Bialystock |
 | The Lion King 1½ (2004) as Timon (voice) |
 | Broadway's Lost Treasures II (2004) as Nathan Detroit (segment "Guys & Dolls") |
 | Timon & Pumbaa Virtual Safari 2.0 (2004) as Timon (voice) |
 | Sesame Street: What's the Name of That Song? (2004) as Self (archive footage) |
| | Timon & Pumbaa's Virtual Safari 1.5 (2004) as Timon (voice) |
 | Behind the Legend: Timon (2004) as Timon (voice) |
 | Win a Date with Tad Hamilton! (2004) as Richard Levy the Driven |
 | Teacher's Pet (2004) as Spot Helperman / Scott Leadready II / Scott Manly-Manning (voice) |
 | Nicholas Nickleby (2002) as Vincent Crummles |
 | Austin Powers in Goldmember (2002) as Mysterious Disco Man |
 | Stuart Little 2 (2002) as Snowbell (voice) |
 | Recording the Producers: A Musical Romp with Mel Brooks (2001) as Self |
 | Laughter on the 23rd Floor (2001) as Max Prince |
 | My Favorite Broadway: The Love Songs (2001) as Self - Perfomer |
 | The Man Who Came to Dinner (2000) as Sheridan Whiteside |
 | Trixie (2000) as Kirk Stans |
 | Titan A.E. (2000) as Preed (voice) |
 | Love's Labour's Lost (2000) as Costard |
 | Isn't She Great (2000) as Irving Mansfield |
 | Stuart Little (1999) as Snowbell (voice) |
 | At First Sight (1999) as Phil Webster |
 | Stuart Little: Making It Big (1999) as Self |
 | Get Bruce! (1999) as Self |
 | The Lion King II: Simba's Pride (1998) as Timon (voice) |
 | Merry Christmas, George Bailey (1997) as Clarence |
 | MouseHunt (1997) as Ernie Smuntz |
| | A Benefit Celebration: A Tribute to Angela Lansbury (1996) as Self |
 | Around the World With Timon & Pumbaa (1996) as Timon 2 (voice) |
 | The Birdcage (1996) as Albert |
 | The Boys Next Door (1996) as Norman Bulansky |
 | The Wizard of Oz in Concert: Dreams Come True (1995) as The Cowardly Lion |
 | Jeffrey (1995) as Father Dan |
 | Circle of Life: An Environmental Fable (1995) as Timon |
 | The Lion King (1994) as Timon (voice) |
 | Addams Family Values (1993) as Desk Sergeant |
 | Life with Mikey (1993) as Ed Chapman |
 | Guys and Dolls: Off the Record (1992) as Himself / Nathan Detroit |
 | The Last Mile (1992) as The Stage Manager |
 | Unnatural Pursuits (1992) as Limo Passenger |
 | Frankie and Johnny (1991) as Tim |
 | He Said, She Said (1991) as Wallace "Wally" Thurman |
 | Joe Versus the Volcano (1990) as Baw - The Waponi Advance Man |
 | The Lemon Sisters (1989) as Charlie Sorrell |
 | Ironweed (1987) as Harold Allen |
 | Alice in Wonderland (1983) as Mouse |
| | Cut Off |
| | Untitled Nathan Lane Documentary as Self |
 | Broadway: The Next Generation as Self |
